Figure 3
From: Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA
7A10B2 scTCR and rFkpA expression level. Western blot showing periplasmic expression levels of 7A10B2 scTCR variants and rFkpA from pHOG, pFKPEN (pEN) and pFKPEI (pEI) vectors under different expression conditions, detected with anti-His tag-HRP and anti-Flag-M2-HRP as described. The scTCR and rFkpA were developed with SuperSignal West Pico substrate and exposed to BioMax MR film for for 30 min and 30 sec, respectively, for the upper and lower panels. The expression analysis was repeated twice, and representative results are shown.