Creation of a novel peptide with enhanced nuclear localization in prostate and pancreatic cancer cell lines

BMC Biotechnology

Table 1 Peptides and Peptide-PNAs Included in Studies

Description	Sequence	Molecular Weight
Glu-FITC	EEE-FITC	776 Da
Glu-Ala-FITC	EEEAA-FITC	919 Da
Glu-Lys-FITC	EEEAAKKK-FITC	1303 Da
Glu-Oct6-FITC	EEEAAGRKRKKRT-FITC	1930 Da
Oct6- FITC	GRKRKKRT-FITC	1400 Da
Phe-Oct6-FITC	FFFAAGRKRKKRT-FITC	1984 Da
Asn-Oct6-FITC	NNNAAGRKRKKRT-FITC	1885 Da
Phe-Oct6-dansyl	FFFAAGRKRKKRT-dansyl	1875 Da
Asn-Oct6-dansyl	NNNAAGRKRKKRT-dansyl	2058 Da
Glu-Oct6	EEEAAGRKRKKRT	1559 Da
Phe-Oct6	FFFAAGRKRKKRT	1613 Da
Tyr-Oct6	YYYAAGRKRKKRT	1661 Da
Tyr-Oct6-FITC	YYYAAGRKRKKRT	2032 Da
Description	Sequence
FITC-PNA-EEE	FITC-TATGATCTCCTCCGT-EEE
FITC-TAT-PNA	FITC-GRKKRRQRRRRC-TATGATCTCCTCCGT
Glu-Oct6-PNA-FITC	EEEAAGRKRKKRT-TATGATCTCCTCCGT-K-FITC
Glu-Oct6-13410a	EEEAAGRKRKKRT-TCCCGTAAATCCCTA
13410a	TCCCGTAAATCCCTA

Carboxyfluorescein and carboxydansyl peptides were synthesized by FMoc chemistry at the Molecular Resources Facility, UMDNJ. They were purified by HPLC and sequence verified by the same technique. Carboxydansyl peptides are synthesized using lysyl dansyl moieties. The peptide-PNAs were synthesized by BioSynthesis; they were purified by HPLC and verified by MALDI-TOF. The letters in bold indicate the PNA portions of the molecules. The FITC moiety was coupled to Glu-Oct6-PNA as a lysyl-FITC, due to the requirements of PNA chemistry.

ISSN: 1472-6750