Figure 4
From: A miniaturized sandwich immunoassay platform for the detection of protein-protein interactions
Comparison of MSIP with resin-based coIP using 18 pairs of interaction candidates identified by YTH. HEK293 cells were cotransfected with FLAG-bait and myc-prey constructs or negative controls. Protein-protein interactions were analyzed by resin-based coIP and MSIP. (A) The samples were probed for interaction by resin-based coIP via western blotting. Stars indicate protein-protein interactions. (B) Samples were probed for PPI by MSIP with 2 slides using monoclonal anti-myc-cy3 (1:200). The slides were scanned by a fluorescence scanner, and the NFI of each PPI was calculated. Flag-X, flag-tag bait; Myc-Y, myc-tag prey; IP, immunoprecipitation; IB, immunoblotting; WB, western blotting; L, lysate.