Figure 1
From: A miniaturized sandwich immunoassay platform for the detection of protein-protein interactions
Overview of the bait-prey approach of MSIP. A 'bait' protein is tagged with FLAG while a 'prey' is tagged with myc. Following transfection and expression of the FLAG- and myc-tagged constructs, cells are lysed and the crude cell lysate is incubated on an anti-FLAG printed aldehyde slide. The FLAG-tagged bait (FLAG-X) is captured by the antibody on the surface of the aldehyde slide, co-capturing any interacting proteins. Myc-tagged prey (myc-Y) interacting with bait is detected by cy3-taged monoclonal anti-myc antibody.