Figure 6
TLR7 activation by hsp65 mRNA. HEK293T cells (3 × 105 cells/well) were co-transfected in triplicate with the TLR expression plasmids. The next day, cells were treated with hsp65 mRNA or following TLRs agonists: poly-IC 100 μg/ml (TLR3), LPS 1 μg/ml (O55:B55) (TLR4) and R-848 1 μg/ml (TLR7 and 8) for 7 hours. The amount of NO release was measured form culture supernatant using the method of Griess. Data are presented as the means ± SD of triplicate cultures. *p < 0.05 were considered significant when compared to empty vector.