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Table 4 Maximum viable cell concentrations in 9 SFM designed by the response surface method.

From: Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design

SFM Maximum viable cell concentration (105 cells/ml)
  Set #1 Set #2 Set #3
SFM* #1 18.12 16.47 9.66
SFM* #2 14.49 20.97 12.87
SFM* #3 22.68 9.45 13.38
SFM* #4 23.13 23.67 17.64
SFM* #5 9.01 9.48 8.61
SFM* #6 10.83 12.48 5.21
SFM* #7 15.21 21.24 16.20
SFM* #8 9.39 6.78 9.04
SFM* #9 12.78 13.20 7.11
  1. Concentrations of cholesterol and polyamine supplement in each SFM are shown in Figure 3A. Three sets of cultures were performed independently with PBMCs obtained from the three different donors. The seeding density was 5 × 105 cells/ml.