Figure 3

Microscopic analysis of cell nuclei. Monolayers of UFL-Ag-286 cells growing in 6 multiwell plates were treated during 48 h both in standard condition (10% FBS) and synchronized condition (1% FBS) in GRACE's medium, and then stained with DAPI. A and B. Synchronized cells show homogeneous morphology of nuclei in an expanded topology (1000×). C and D. Non-synchronized cells show heterogeneous morphology with evidence of mitotic events, marked with white arrow (1000×).