Growth of UFL-Ag-286 cells in culture conditions with different proportions of FBS. Monolayers of UFL-Ag-286 cells cultivated in 96 multiwell plates for 48 h were treated with four different concentrations of FBS (0.5, 0.75, 1.0 and 10%) in GRACE's medium. Thereafter, the total number of cells was measured by a colorimetric method (A), and the cell viability was measured by a MTT method (B). For each treatment, data were relativized to the respective 0 h time. Then, the obtained values were normalized with respect to the maximum value. The condition with 1.0% of FBS was not toxic to the cell culture (p = 0.1693, Student's T test), below this concentration the cellular viability was affected (***P < 0.001, *P < 0.05, Student's T test).