Figure 5
From: Stabilizing mutations increase secretion of functional soluble TCR-Ig fusion proteins
Effect of V and C region mutations on secretion and folding of TCRV-IgC and cTCR-IgFc molecules produced in insect cells. Secretion of 4B2A1 derived TCRV-IgC fusion proteins with or without V region mutations. One representative experiment out of three is shown (A). Secretion of 4B2A1 derived cTCR-IgFc fusion proteins with or without V and/or C region mutations. One representative experiment out of three is shown (B). Recognition by anti-TCR 4B2A1 specific Abs. One representative experiment out of two is shown. TCR domains are filled, and Ig domains open circles (C). Insect cell supernatant after three rounds of amplifications was tested in triplicates. Error bars: SD.