Figure 3

Effect of V region mutations on secretion and folding of TCRV-IgC molecules of the (V _α H+V _β L) format. Secretion of wild type (wt) and mutant 4B2A1- and 7A10B7-derived fusion proteins (4V mut and 7V mut) after transfection of HEK 293E cells. One representative experiment out of five is shown (A). Recognition by anti-TCR Abs (B). Wt and mutant 4B2A1 derived Ig-fusions were tested in ELISA using two Abs that recognize the 4B2A1 receptor (GB113 and F23.2) and H57, which is C region specific. One representative experiment out of three is shown (B and C). Wt and mutant 7A10B2 derived Ig-fusions were tested in ELISA using two Abs that recognize the 7A10B2 receptor (44-22-1 and RR4-7) and H57. All molecules were analyzed at the same concentration of 0.012 μg/ml. One representative experiment out of three is shown (C). Cell supernatant was tested in triplicates. Error bars: SD