Figure 2
From: Stabilizing mutations increase secretion of functional soluble TCR-Ig fusion proteins
Secretion of fusion proteins. The four molecules to the left are derived from 4B2A1 (abbreviated 4), the four to the right from 7A10B2 (abbreviated 7). The TCR Vα domain was fused to the heavy chain C region and the TCR Vβ domain on the light chain C region (VαH + VβL) or vice versa in the TCRV-IgC format. The complete TCR α chain was fused to the complete heavy chain and the TCR β chain fused to the light chain (αH + βL) or vice versa in the cTCR-cIg format. TCR domains are filled, Ig domains are open circles. Cell supernatant was tested in triplicates. Error bars: Standard deviation (SD). One representative experiment out of three is shown.