Oligonucleotide indexing of DNA barcodes: identification of tuna and other scombrid species in food products

BMC Biotechnology

Table 3 Application of the described methods: species discrimination of tuna and scomber species in food product.

*Sample*	*Species Declared*	*N° of samples/batch*	*Species identified (fragment AB)*
Canned tuna		10	10 T. albacares
		10	9 T. albacares
			1 T. thynnus
		10	10 T. albacares
	T. albacares	100	2 T. albacares
			72 T. obesus
			5 K. pelamis
			19 mix T. obesus/K. pelamis
			2 mix T. albacares/T. obesus
		10	10 T. albacares
		10	10 T. albacares
		10	10 T. albacares
		10	9 T. albacares
			1 new variant (Thunnus spp.)
		10	9 T. albacares
			1 new variant (Thunnus spp.)
		8	8 T. albacares
		1	1 T. obesus
		2	2 T. albacares
Tuna salad	T. albacares	2	() 2 K. pelamis*
Tuna sauce (20% tuna)	T. albacares	8	() Mix of species* (different genera)
Canned scomber	S. japonicus	2	2 S. colias

(*) Only fragment B
A total of 203 samples of processed food were delivered in 15 batches by industrial retailers for the control of species content and analyzed for polymorphism in fragment AB of CytB.

ISSN: 1472-6750