Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

Table 1 Comparison of expression levels and N-glycan composition of SEAP in Sf9, High Five, and Ao38 cells

Cell line	Sf9	High Five	Ao38
Expression level (U/ml)	6	11.6	23.2
Identified glycans	Man2, Man3, Man3F, Man4, Man5-8	Man2, Man3, Man4, Man4F, Man5-8	Man2F, Man3F, Man4F, Man3F1,2
			Man2-6, HexNAc-Man3
	Man3F: 30%	Man3: 25%	Man3F: 53% for N¹³⁹
Major component^a	Man4: 40%	Man4F: 15%	Man6: 31% for N²⁶⁶
			Man3: 29% for N²⁶⁶

^a Percent composition of the major component of N-glycans were determined by LC/MS/MS for each glycan (N139 or N266) of Ao38-expressed SEAP, and compared with published data from total glycans of SEAP expressed in Sf9 and High Five cells (see reference [10]).

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