RT-PCR analysis and TNCL virus detection. (A) To examine cell lines for the presence of a TNCL virus, five primer pairs were used for RT-PCR analysis of total RNA from cell lines. Ao38, AoP (passage 5), Sf9, and High Five cells were infected with AcMNPV 48 h prior to RNA isolation. The relative locations of primer pairs on TNCL Virus RNAs 1 and 2 are indicated in the upper diagram, and primer sequences are listed in Table 2. Primer sets were labeled as follows: Set 1, Noda-R1-190F and Noda-R1-636R; Set 2, Noda-R1-1093F and Noda-R1-1531R, Set 3; Noda-R1-2368F and Noda-R1-2933R; Set 4, Noda-R2-269F and Noda-R2-810R; Set 5, Noda-D4 and Noda-U4. Primer sets and cell lines are indicated above each lane, and sizes (Kbp) of marker DNAs are indicated on the left of each gel.(B). RT-PCR analysis of gp64 transcript RNA control. RT-PCR (left) and PCR (right) were used to analyze total RNA from Ao38, Ao-P (passage 5), Sf9, and High Five cells infected with WT AcMNPV, AcMNPV gp64 primers are listed in Table 2. Samples treated with DNaseI (+) or not treated with DNaseI (-) are indicated above lanes. Sizes (kbp) of marker DNAs are indicated on the left.