Figure 4

Ao38 cell volumes, AcMNPV susceptibility, and virus yields. (A) Measurements of packed cell volumes (PCV, μl per 3 × 10⁶ cells) of Ao38, Sf9 and High Five cells are compared in a bar graph. Gray bars represent averages and standard deviations from 3 replicate measurements are indicated. (B) Susceptibility of Ao38, Sf9, and High Five cells to AcMNPV infection. The relative susceptibilities of Ao38, Sf9, and High Five cells to AcMNPV infection was measured by infecting each cell line with an AcMNPV virus (βgal-AcMNPV, 1 × 10⁷ IU/ml) that was initially titred on Sf9 cells. The same virus preparation (βgal-AcMNPV) was then titred on each cell line. Gray bars represent averages of virus titers determined on each cell line at 4 days p.i. and standard deviations of triplicate infections are indicated. (C) Comparisons of WT AcMNPV production from Sf9, High Five, and Ao38 cells in TNMFH medium, and from Ao38 cells in Sf-900III serum-free medium. Virus titres were determined from the supernatants of triplicate infections of each cell line with WT AcMNPV at various times post infection (Y-axis).