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Figure 3 | BMC Biotechnology

Figure 3

From: Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

Figure 3

Ct values according to different extraction methods for paired fresh-frozen and FFPE tissues. Comparison of the summary statistics (mean Ct, standard deviation, and overall range of Ct values) using raw Ct scores obtained from the normal lymph nodes and mantle cell lymphoma samples extracted using three separate RNA extraction methods. On comparison of the FFPE summary statistics to those from the mirVana and TRIzol-Qiagen extraction protocols, we see that the mean Ct values obtained from the FFPE samples are significantly higher than those obtained from the mirVana and TRIzol-Qiagen extraction protocols (p < 0.0001 and p < 0.0001 respectively).

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BMC Biotechnology

ISSN: 1472-6750

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