Table 1 Validation of Tm determinations for antiparallel duplex and parallel triplex formations
From: A novel FRET pair for detection of parallel DNA triplexes by the LightCycler
Mean (°C) | Standard Deviation | Number | Minimum (°C) | Maximum (°C) | CV% | |
|---|---|---|---|---|---|---|
Antiparallel duplex | ||||||
Intra assay | 73.60 | 0.14 | 12 | 73.33 | 73.80 | 0.19 |
Inter assay | 73.65 | 0.09 | 6 | 73.52 | 73.80 | 0.12 |
Parallel triplex | ||||||
Intra assay | 75.03 | 0.08 | 12 | 74.89 | 75.13 | 0.11 |
Inter assay | 75.11 | 0.10 | 6 | 75.01 | 75.25 | 0.14 |