Figure 3
From: A novel FRET pair for detection of parallel DNA triplexes by the LightCycler
Melting curves demonstrating pH dependence of antiparallel duplex and parallel triplex FRET-pairs. All experiments in sodium cacodylate buffer with MgCl2. (A, B) Fluorescence of ATTO495-647N and FAM-Cy5 antiparallel duplex (AD) FRET pairs at 670 nm from pH 5.5 to 7.5 (0.5 μM of each probe). (C, D) Fluorescence of ATTO495-ATTO647N and FAM-Cy5 parallel triplex (PT) FRET pairs at 670 nm from pH 4.5 to 6.0 (1.0 μM of each probe). (E) Fluorescence of FAM-BHQ1 parallel triplex at 530 nm from pH 4.5 to 6.0 with 1.0 μM of each probe.