Figure 1

Transfection efficiency of hUCB-MSCs. (A) pEGFP-N1 vector was transfected into hUCB-MSCs by the established electroporators Nucleofector^®, ECM 830, MicroPulser™, and a novel electroporator, MicroPorator™, or by a liposome-based reagent, Gene Porter2. After 24 hr, EGFP expression was analyzed using phase contrast and fluorescence microscopy. (B) Transient expression of EGFP was assessed by FACS analysis. (C) After transfection, cells were cultured for additional 48 hr and analyzed for viability by the MTS assay. The data are expressed as the mean ± SEM; n = 3. Scale bar = 200 μm.