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Table 1 Helper oligonucleotides (chaperones) used in the current study and their characteristics.

From: Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides

  Position Length Sequence 5'-3' GC% Tm ΔG
ChpA 33-47 15 AGTCGCAAAATATGC 40 53,8 -21,0
ChpB 52-64 13 GTTTACGTCGCCA 53,8 54,2 -20,0
ChpC 116-129 14 CCTGCTGGTTTTTA 42,9 56,9 -19,6
ChpD 131-143 13 CAAATCGGGTCAC 53,8 54 -20,1
ChpE 188-202 15 TAGACAAGGCTTAAT 33,3 54,6 -20,5
ChpF 248-261 14 CCCTCGACACATAA 50 62,5 -21,6
  1. Tm and ΔG are calculated by program SLICSEL.