Table 1 Helper oligonucleotides (chaperones) used in the current study and their characteristics.
From: Detection of tmRNA molecules on microarrays at low temperatures using helper oligonucleotides
Position | Length | Sequence 5'-3' | GC% | Tm | ΔG | |
|---|---|---|---|---|---|---|
ChpA | 33-47 | 15 | AGTCGCAAAATATGC | 40 | 53,8 | -21,0 |
ChpB | 52-64 | 13 | GTTTACGTCGCCA | 53,8 | 54,2 | -20,0 |
ChpC | 116-129 | 14 | CCTGCTGGTTTTTA | 42,9 | 56,9 | -19,6 |
ChpD | 131-143 | 13 | CAAATCGGGTCAC | 53,8 | 54 | -20,1 |
ChpE | 188-202 | 15 | TAGACAAGGCTTAAT | 33,3 | 54,6 | -20,5 |
ChpF | 248-261 | 14 | CCCTCGACACATAA | 50 | 62,5 | -21,6 |