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Table 3 Effect of glycerol, phosphate and incubation temperature after IPTG addition on mBCO expression.

From: Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli

Medium Temperature after induction
(°C)
Biomass
(g/Lculture)
Specific activity
(U/mgprotein)
Volumetric productivity
(U/Lculture)
LB 18 8.7 ± 1.9 1.3 ± 0.3 770 ± 130
  25 6.8 ± 0.7 1.7 ± 0.3 860 ± 80
  37 5.3 ± 0.5 ≤ 0.01 3.0 ± 0.3
LB+G 25 12.7 ± 1.3 0.87 ± 0.10 760 ± 80
  37 10.8 ± 1.1 0.08 ± 0.01 65 ± 7
TB 18 18.7 ± 3.3 3.8 ± 0.6 8030 ± 1400
  25 24.3 ± 2.6 3.9 ± 1.0 11100 ± 1100
  37 22.7 ± 2.4 ≤ 0.01 9.0 ± 0.9
TB-Pi 25 28.3 ± 3.1 4.1 ± 1.1 13100 ± 1300
TB-G 25 18.0 ± 1.0 3.0 ± 0.5 6140 ± 200
  37 6.0 ± 0.3 ≤ 0.01 7.0 ± 0.2
TB+4G 25 22.4 ± 2.5 2.8 ± 0.5 11000 ± 400
TB+G 25 24.3 ± 2.6 3.9 ± 0.9 11200 ± 1100
TB+20G 25 21.9 ± 1.5 5.9 ± 1.0 14200 ± 2800
TB-Pi-G 25 19.8 ± 1.1 2.8 ± 0.5 8000 ± 260
SB 25 18.8 ± 0.7 4.1 ± 0.2 7900 ± 140
SB+Pi 25 19.5 ± 1.4 3.5 ± 1.1 8720 ± 2340
SB+G 25 22.5 ± 1.6 4.6 ± 0.8 11100 ± 1500
SB+G+Pi 25 19.2 ± 1.3 5 ± 1 10300 ± 1700
  1. E. coli cells carrying the recombinant pET24Δ-mBCO plasmid were grown at 37°C, BCO expression was induced by 1 mM IPTG at OD600 nm = 1.5-2 and harvested after 18 hours at different temperatures. G: glycerol. +G, +Pi: media added of glycerol (8 mL/L) and/or phosphate salts (70 mM), respectively; +4G and +20G: media added of 4 mL/L and 20 mL/L glycerol, respectively; -G, -Pi: media deprived of glycerol and/or phosphate salts, respectively. Data are expressed as mean ± S.D., n = 3.