Table 3 Effect of glycerol, phosphate and incubation temperature after IPTG addition on mBCO expression.
From: Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli
Medium | Temperature after induction (°C) | Biomass (g/Lculture) | Specific activity (U/mgprotein) | Volumetric productivity (U/Lculture) |
|---|---|---|---|---|
LB | 18 | 8.7 ± 1.9 | 1.3 ± 0.3 | 770 ± 130 |
25 | 6.8 ± 0.7 | 1.7 ± 0.3 | 860 ± 80 | |
37 | 5.3 ± 0.5 | ≤ 0.01 | 3.0 ± 0.3 | |
LB+G | 25 | 12.7 ± 1.3 | 0.87 ± 0.10 | 760 ± 80 |
37 | 10.8 ± 1.1 | 0.08 ± 0.01 | 65 ± 7 | |
TB | 18 | 18.7 ± 3.3 | 3.8 ± 0.6 | 8030 ± 1400 |
25 | 24.3 ± 2.6 | 3.9 ± 1.0 | 11100 ± 1100 | |
37 | 22.7 ± 2.4 | ≤ 0.01 | 9.0 ± 0.9 | |
TB-Pi | 25 | 28.3 ± 3.1 | 4.1 ± 1.1 | 13100 ± 1300 |
TB-G | 25 | 18.0 ± 1.0 | 3.0 ± 0.5 | 6140 ± 200 |
37 | 6.0 ± 0.3 | ≤ 0.01 | 7.0 ± 0.2 | |
TB+4G | 25 | 22.4 ± 2.5 | 2.8 ± 0.5 | 11000 ± 400 |
TB+G | 25 | 24.3 ± 2.6 | 3.9 ± 0.9 | 11200 ± 1100 |
TB+20G | 25 | 21.9 ± 1.5 | 5.9 ± 1.0 | 14200 ± 2800 |
TB-Pi-G | 25 | 19.8 ± 1.1 | 2.8 ± 0.5 | 8000 ± 260 |
SB | 25 | 18.8 ± 0.7 | 4.1 ± 0.2 | 7900 ± 140 |
SB+Pi | 25 | 19.5 ± 1.4 | 3.5 ± 1.1 | 8720 ± 2340 |
SB+G | 25 | 22.5 ± 1.6 | 4.6 ± 0.8 | 11100 ± 1500 |
SB+G+Pi | 25 | 19.2 ± 1.3 | 5 ± 1 | 10300 ± 1700 |