Figure 5

Transduced αT2ib inhibits surface TLR2 expression by murine macrophages. A-C, Flow cytometry of cell surface TLR2 of HEK293 cells overexpressing mouse TLR2 (A), RAW264.7 cells (B, Black area, PE staining correlating with TLR2 expression; white area, PE staining mediated by isotype control antibody; living infected cells were gated on GFP), and peritoneal macrophages (C) expressing αT2ib. Inset in left diagramm represents viability of primary macrophages (ethidium monoazide staining detected in PE-Texas Red versus PE channels, double positive cells excluded; grey line in main diagrams, no primary mAb, -1. Ab; black line in main diagrams, PE-labelled αTLR2 mAb T2.5).