Expression of adenovirally transduced αT2ib in RAW264.7 macrophages. A, For myc-tag specific immunoblot analysis of RAW264.7 cell lysate the αT2ib protein band was visualized by application of a mouse anti-myc antibody. Non infected (no inf.) and AdVGFP infected cells were used as control. B, Transduction efficiency of RAW264.7 cells infected with AdVGFP or AdVαT2ib analysed by flow cytometry. Portion of infected cells was determined by analysis of green fluorescence by flow cytometry after 3 days of infection (empty area, noninfected cells; black area, cells infected as indicated). C, D, Immunofluorescence analysis by microscopy of permeabilized and fixed cells (i, Transmitted light; ii, Expression of EGFP visualized by UV-light; iii, Analysis of myc expression using a secondary TRITC labelled antibody).