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Table 3 PCR screening results of transgenic insect cell lines.

From: Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

Construct Product 1 Product 2 Product 3 gag Neo Amp
pXLNeoGag passage 15 in Sf21 cells + + + + + +
pXLNeoGag passage 26 in Sf21 cells + + + + + +
pXLNeoGag passage 23 in Sf9 cells X + + + + +
   passage 7     +   
   passage 13     +   
   passage 93     +   
pXLIE-SVGag passage 16 in Sf21 cells X + X + X +
pXlHr3ieGag passage 9 in Sf21 cells X + X + X +
pXLHSP70Gag passage 6 in Sf21 cells X + X + X +
  1. Screening was carried out using primers complementary to different sections of the pXLBacIINeoGag vector. The presence of a PCR product is indicated with a plus (+) and absence with a cross (X). Confirmation of stable integration up to 93 passages was indicated by performing PCR amplification on several passages of pXLNeoGag in Sf9 cells (see bold font).