Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

BMC Biotechnology

Table 3 PCR screening results of transgenic insect cell lines.

Construct	Product 1	Product 2	Product 3	*gag*	Neo	Amp
pXLNeoGag passage 15 in Sf21 cells	+	+	+	+	+	+
pXLNeoGag passage 26 in Sf21 cells	+	+	+	+	+	+
pXLNeoGag passage 23 in Sf9 cells	X	+	+	+	+	+
passage 7				+
passage 13				+
passage 93				+
pXLIE-SVGag passage 16 in Sf21 cells	X	+	X	+	X	+
pXlHr3ieGag passage 9 in Sf21 cells	X	+	X	+	X	+
pXLHSP70Gag passage 6 in Sf21 cells	X	+	X	+	X	+

Screening was carried out using primers complementary to different sections of the pXLBacIINeoGag vector. The presence of a PCR product is indicated with a plus (+) and absence with a cross (X). Confirmation of stable integration up to 93 passages was indicated by performing PCR amplification on several passages of pXLNeoGag in Sf9 cells (see bold font).

ISSN: 1472-6750