Table 2 Primer sequences used in the screening of transgenic insect cell lines expressing Gag protein.
Primer pair | Product |
|---|---|
sense CTAAATTGTAAGCGTTAATATTTTG antisense GGTAGCGACCCCCTCAGCCAATAAGAATTC | Product 1 (3'ITR and 3' end of gag) |
sense: GGGTTAATCTAGCTGCATCAGGATC antisense: GAGCGCACGAGGGAGCTTC | Product 2 (5' ITR) |
sense: CTGGGATGGGTGGGTTGCTGG antisense:CTTCTGAGAGCTCGATCGATAC | Product 3 (Junction between neomycin and gag) |
sense ATGGGTGCTCGCGCATCTATCC antisense ATTCTTGGCTGAGGGGGTCGC | Gag |
sense GCGGTACCATGATTGAACAAG antisense CGGAGCTCTCAGAAGAACTCG | Neomycin |
sense CAGTTACCAATGCTTAATCAG antisense GTATGAGTATTCAACATTTCCG | Ampicillin |