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Figure 1 | BMC Biotechnology

Figure 1

From: Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy

Figure 1

Tol2 based luciferase reporter constructs to read-out circadian molecular oscillation in mammalian cells. Bmal1:luc-pT2A construct contains 0.5 kb-long mouse Bmal1 promoter driven luciferase cDNA and hygromycin B-resistant cassette (Hyg+). The Dbp:luc-pT2A construct contains hygromycin B-resistant cassette (Hyg+) and 0.6 kb-long mouse Dbp promoter driven luciferase cDNA (see Methods).

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BMC Biotechnology

ISSN: 1472-6750

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