Figure 6
Immunoblot validation of identified protein spots. A western analysis of biological triplicate samples with c-Myc over-expression observed in the cMycCHO cell lines validated the observed proteomic results. The membranes were stained with Coomassie brilliant blue to re-verify loading and uniformity of the electro-transfer after band detection, due to c-myc de-regulating both cytoskeleton and metabolic proteins.