Figure 4

Endostatin is present in the stroma adjacent to the device. (A and B) Immunohistochemical analyses using BSA in the tissue around the devices with (A) LM(pSecTag) cells; (B) LM (pSecTag-end) cells, showing the absence of reaction in the stroma adjacent to the devices; (C and D)- Immunohistochemical analyses using endostatin antibody of the environment around the device; (C) Immunohistochemical analyses from mice with melanoma tumor treated with encapsulated LM(pSecTag) cells, sustaining that there is no endostatin in the tissues adjacent to the device; (D) Immunohistochemical analyses from mice with melanoma tumor treated with encapsulated LM(pSecTag-end) cells, indicating the presence of endostatin in the stroma adjacent to the device which was implanted with endostatin-expressing cells. The reaction that shows up in the cells inside the device is due to an unspecific binding. The reaction in the adjacent tissue is due to the presence of endostatin . (i)- region inside the device; (m)- membrane; (o)- region outside the device.