Skip to main content

Table 2 CFUs of recombinant strains TYEGALLAC3-6 on induction and non-induction media.

From: Heterologous expression of a tannic acid-inducible laccase3 of Cryphonectria parasitica in Saccharomyces cerevisiae

Strain

CFUs/plate1

 

YEPD2

ura-/Gal

Mock transformant

  

   YEPD plating

792 ± 28 3

749 ± 11

   ura- plating

763 ± 13

711 ± 23

TYEGALLAC3-6

  

   YEPD plating

768 ± 15

391 ± 21

   ura- plating

690 ± 17

175 ± 15

  1. 1 Colony-forming units per plate
  2. 2 For each strain, 1 × 107 cells were inoculated into corresponding media, as described in Materials and methods. YEPD and galactose-supplemented glucose free ura- selective media (ura-/Gal) were used as non-induction and induction media, respectively.
  3. 3 Five replicates of each strain were used, and each experiment was repeated three times. Values are shown as the means ± standard deviation.