Figure 1

Expression of laccase3 from recombinant S. cerevisiae TYEGLAC3-1 and TYEGALLAC3-6. (A) Brown coloration of DMOP substrate by the recombinant laccase3 in the culture supernatant of TYEGLAC3-1. Colormetric detection after 1 h in a reaction mixture is shown. Numbers above each well indicate the number of days after cultivation. Results of vector-only transformants are shown at corresponding lower lines of wells as a control. Cell growth (dotted line) and laccase3 activity (solid line) in the culture filtrate of recombinant S. cerevisiae TYEGLAC3-1 (B) and TYEGALLAC3-6 (C) at various days after cultivation. YEPD (non-induction) and galactose-supplemented glucose-free ura^- (induction) media are indicated after the strain designation as NI and I, respectively. Cell growth was monitored by measuring OD₆₀₀ with a spectrophotometer. Vector-only transformed (Mock) cells were included as a control.