Figure 5

One-Component System. (A) Schematic diagram of one-component molecular switch system. All components of the molecular switch system were cloned into a pMSCV vector with eGFP as the target gene called GRQCIMFIGFP. (B, C, and D) HEK293T cells were transfected with GRQCIMFIGFP and (B) no ligand (C) 10 nM, or (D) 10 μM of LG335. Pictures were taken 40 hours after transfection and 32 hours after adding ligand.