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Figure 3 | BMC Biotechnology

Figure 3

From: Characterization of a molecular switch system that regulates gene expression in mammalian cells through a small molecule

Figure 3

GRQCIMFI Orthogonality. Luciferase assays in the presence of 1 μM different ligands: LG335, 9cRA, or atRA. Constitutively expressed nuclear receptor, GRQCIMFI or RXRwt were cotransfected with p17*4TataLuc or pLuc_CRBPII. These plasmids have either Gal4 or RXR response elements respectively as well as the target gene, luciferase. Fold inductions for this assay are reported and normalized to wells with no ligand.

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BMC Biotechnology

ISSN: 1472-6750

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