Table 1 PCR primers for amplifying the SUMO-FGF21 fusion gene
Primer name (size) | Sequence (5'- 3) |
|---|---|
Pa(32 bp) | ggaattccatatgcatcatcatcatcatcacg |
Sumo linker(39 bp) | tggagtcagggatggggtgaccaccaatctgttctctgt |
P1(59 bp) | gccggatccatgcaccccatccctgactccagtcctctcctgcaattcgggggccaagt |
P2(59 bp) | ctgtctgctgggcatcatctgtgtagaggtaccgctgccggacttgcccccg aattgc |
P3(59 bp) | gatgatgcccagcagacagaagcccacctggagatcagggaggatgggacggtgggggg |
P4(59 bp) | tttcagctgcaggagactttcggggctctggtcagcagcgccccccaccgtcccatcct |
P5(59 bp) | aagtctcctgcagctgaaagccttgaagccgggagttattcaaatcttgggagtcaaga |
P6(59 bp) | tacagggccccatctggccgctggcacaggaacctggatgtcttgactcccaagatttg |
P7(59 bp) | ggccagatggggccctgtatggatcgctccactttgaccctgaggcctgcagcttccgg |
P8(59 bp) | cggactggtaaacattgtatccgtcctcaagaagcagctcccggaagctgcaggcctca |
P9(59 bp) | tacaatgttaccagtccgaagcccacggcctcccgctgcacctgccagggaacaagtc |
P10(59 bp) | gaagcgagctggtcctcggggtgcagggtcccggtgtggggacttgttccctggcaggt |
P11(59 bp) | ccgaggaccagctcgcttcctgccactaccaggcctgccccccgcactcccggagccac |
P12(59 bp) | gaggagcccacatcggggggctggggggccaggattccgggtggctccgggagtgcggg |
P13(59 bp) | cccccgatgtgggctcctcggaccctctgagcatggtgggaccttcccagggccgaagc |
P14(46 bp) | ccgctcgagtcaggaagcgtagctggggcttcggccctgggaaggt |