Figure 1From: High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coliSchematic illustration of SUMO-FGF21 synthesis. According to the human FGF21 mRNA coding sequence and the SUMO fragment C-terminal sequence, 21 primers were designed and synthesized. As shown in Figure 1, the SUMO-FGF21 was synthesized by primer extension and PCR. The detailed steps are reported in Material and Methods.Back to article page