A) Schematic depicting in vivo assay system for gene silencing in tick nymphs and eggs. All RNAi experiments using candidate tick gene dsRNA followed the same procedure. B: A schematic transdermal delivery of dsRNA by electroporation in tick nymphs. A) Unfed nymphs trapped in dsRNA and, B) electric pulse discharged through electrodes. a) Arrow indicates the size of unfed nymph, b) schematics theoretical influx of labeled long dsRNA in the unfed nymphs and, c) partially feed nymphs after feeding on mouse.