A surrogate-based approach for post-genomic partner identification

BMC Biotechnology

Table 5 Results of panning experiments on RNA targets using secondary libraries

Target	Primary	Secondary	Target Specific	Target Specific
	Binder	Binder (%)	Binders: Primary	Binders: Secondary
	(%)		(%)	(%)
APP	78	95	9	40
HCV	86	98	13	17
IGF-1	77	99	4	18

Clones were categorized as specific binders if they had a binding ratio of target:irrelevant ≥ 2.0, as described in Table 2 above. Comparison of percentage specific binders from pannings of primary libraries (expressing random peptides) vs. secondary libraries (based on target-specific primary surrogates) is shown in this Table.

ISSN: 1472-6750