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Table 1 Preparation and properties of polystyrene ball modified with copolymers of acrylamide and methacryloyl hydrazide

From: Oriented antibody immobilization to polystyrene macrocarriers for immunoassay modified with hydrazide derivatives of poly(meth)acrylic acid

Copolymer

4-Nitro-

Nitrogen content

Copolymer

Content of active hydrazide groups on

 

phenyl

in the copolymer

activity by

a carrier (μmol/cm2), determined

 

acrylate

by elemental

the reaction

  
    

by the decrease in the

by the reaction

 

content,

analysis, %

with TNBS*,

  
    

copolymer content in

of ball with

 

mol.%

(theoretical

OD520

  
    

solution

TNBS

  

value)

   

CAMAH-1

10

19.1 (20.8)

0.39

0.42

0.06

CAMAH-2

33

24.6 (23.1)

1.42

1.23

0.31

CAMAH-3

50

24.5 (24.5)

1.28

1.05

0.31

  1. * 0.2 ml of a copolymer solution (0.1 mg/ml) was added to 1 ml of 2.5 · 10-3 M TNBS solution in 0.1 M borax. The solution obtained was allowed to stay for 15 min, and then OD520 was measured.