The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle

BMC Biotechnology

Table 2 Experiment I. Immunoglobulin in mice immunized according to the experimental group^a.

Immunogen / adjuvant	IgG1	IgG2a	IgG2b	Total IgG
Bm86 / Montanide 888	0.519 (71.39) ^b	0.128 (17.61)	0.080 (11.00)	0.727
Bm86 / cells	0.271 (60.90)	0.094 (21.12)	0.080 (17.98)	0.445
Bm86 / membrane	0.566 (74.57)	0.127 (16.73)	0.066 (8.70)	0.759
Bm86 / membrane / saponin	0.637 (54.17)	0.465 (39.54)	0.074 (6.29)	1.176
Cells	0.278 (66.03)	0.083 (19.72)	0.060 (14.25)	0.421
Membrane	0.498 (70.14)	0.170 (23.94)	0.042 (5.92)	0.710
Membrane / saponin	0.717 (67.64)	0.251 (23.68)	0.92 (8.68)	1.060

^a Ten mice per group were immunized with 5 μg Bm86 (except the controls) adjuvated as indicated. Antibody levels were expressed as the absorbance at 280 nm of the pooled serum samples at day 40 (Garcia - Garcia et al., 1995). b Within the parentheses the percent of the total IgG that corresponds to the subclass.

ISSN: 1472-6750