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Table 1 Experiment I. Anti-Bm86 levels in mice immunized according to the experimental groupsa.

From: The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle

 

Days

Immunogen / adjuvant

10

20

30

40

Bm86 / Montanide 888

0.23 ± 0.24

1.34 ± 0.58

2.17 ± 0.54

1.02 ± 0.12

Bm86 / cells

0.17 ± 0.05

0.16 ± 0.10

0.24 ± 0.31

0.25 ± 0.07

Bm86 / membrane

0.17 ± 0.07

1.20 ± 0.67

2.09 ± 0.48

1.09 ± 0.27

Bm86 / membrane / saponin

0.23 ± 0.09

2.16 ± 0.39

2.68 ± 0.18

1.09 ± 0.12

Cells

0.15 ± 0.07

0.07 ± 0.05

0.18 ± 0.06

0.19 ± 0.05

Membrane

0.22 ± 0.08

0.19 ± 0.11

0.26 ± 0.11

0.26 ± 0.14

Membrane / saponin

0.21 ± 0.06

0.12 ± 0.03

0.23 ± 0.08

0.25 ± 0.11

  1. a Ten mice per group were immunized with 5 μg Bm86 adjuvated as indicated (Garcia - Garcia et al., 1995). ELISA was used to determine total antibody response against Bm86.Briefly, 100 μl of antiserum diluted 1: 100 in PBS were added to the wells, incubated for 2 h at 37°C, washed four time with PBST and developed with HRP-conjugated goat anti-mouse IgG as describe in material and methods. Antibody titres were expressed as the mean absorbance value (O.D.492nm) per group ± SD (N = 10).
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BMC Biotechnology

ISSN: 1472-6750

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