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Table 1 Experiment I. Anti-Bm86 levels in mice immunized according to the experimental groupsa.

From: The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle

  Days
Immunogen / adjuvant 10 20 30 40
Bm86 / Montanide 888 0.23 ± 0.24 1.34 ± 0.58 2.17 ± 0.54 1.02 ± 0.12
Bm86 / cells 0.17 ± 0.05 0.16 ± 0.10 0.24 ± 0.31 0.25 ± 0.07
Bm86 / membrane 0.17 ± 0.07 1.20 ± 0.67 2.09 ± 0.48 1.09 ± 0.27
Bm86 / membrane / saponin 0.23 ± 0.09 2.16 ± 0.39 2.68 ± 0.18 1.09 ± 0.12
Cells 0.15 ± 0.07 0.07 ± 0.05 0.18 ± 0.06 0.19 ± 0.05
Membrane 0.22 ± 0.08 0.19 ± 0.11 0.26 ± 0.11 0.26 ± 0.14
Membrane / saponin 0.21 ± 0.06 0.12 ± 0.03 0.23 ± 0.08 0.25 ± 0.11
  1. a Ten mice per group were immunized with 5 μg Bm86 adjuvated as indicated (Garcia - Garcia et al., 1995). ELISA was used to determine total antibody response against Bm86.Briefly, 100 μl of antiserum diluted 1: 100 in PBS were added to the wells, incubated for 2 h at 37°C, washed four time with PBST and developed with HRP-conjugated goat anti-mouse IgG as describe in material and methods. Antibody titres were expressed as the mean absorbance value (O.D.492nm) per group ± SD (N = 10).