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Table 1 Carbon source utilization by β7328 and descendents

From: Long term adaptation of a microbial population to a permanent metabolic constraint: overcoming thymineless death by experimental evolution of Escherichia coli

  Strains and generations
Substrates β7328 γ1710 γ3330 γ5010 γ8094
- - - - - -
Mannose + + + + -
D(+) Galactose + + + - -
1-0-Methyl-β-Galactopyranoside + + + - -
m-Coumarate + + + - -
1-0-Methyl-β-glucopyranoside + - - - -
α-L-(-) Fucose + - - - -
Mucate + - - - -
L(+)-Tartrate + - - - -
L(-)-malate + - - - -
D(+)-Malate + - - - -
5-Keto-D-gluconate + - - - -
Succinate + - - - -
DL-Glycerate + - - - -
L-Aspartate + - - - -
L-Proline + - - - -
L-Alanine + - - - -
L-serine + - - - -
propionate + - - - -
α-Ketoglutarate + - - - -
Calculated pathway inactivation probability by genetic drift   0.085–0.85% 0.079–0.79% 0.082–0.82% 1.02%
Observed pathway inactivation probability (under assumption of independence)   15/100 15% 0/85 0% 3/85 3.5% 1/82 1.2%
  1. Growth on 100 different carbon sources was monitored using API100™ kits (Bio-merieux). Observed phenotypes are designated by +(growth) and – (no growth). The mutation probabilities were calculated as follows: an average pathway for carbon source utilization contains from 1 to 10 pathway specific genes (10 to 104 bp). The mutation rate per bp per generation in E. coli is 5.4 10-10[30] and if the assumption is made that every mutation is inactivating (which is the most conservative hypothesis), the probability of acquiring a mutation in a given pathway after X generations by genetic drift is X 5.4 10-10 103 or 4. As the mannose-deficient-phenotype can be due to a mutation in manA, manXYZ or, nagC corresponding to 4128 bp [31], this value was used for the mutation probability between γ5010 and γ8094.