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  1. Research article

    Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 β-N-acetylglucosaminidase and salt tolerance

    Enzymatic degradation of chitin has attracted substantial attention because chitin is an abundant renewable natural resource, second only to lignocellulose, and because of the promising applications of N-acety...

    Junpei Zhou, Zhifeng Song, Rui Zhang, Rui Liu, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han and Zunxi Huang

    BMC Biotechnology 2017 17:37

    Published on: 11 April 2017

  2. Research article

    Improved n-butanol production via co-expression of membrane-targeted tilapia metallothionein and the clostridial metabolic pathway in Escherichia coli

    N-Butanol has favorable characteristics for use as either an alternative fuel or platform chemical. Bio-based n-butanol production using microbes is an emerging technology that requires further development. Al...

    Wei-Chih Chin, Kuo-Hsing Lin, Chun-Chi Liu, Kenji Tsuge and Chieh-Chen Huang

    BMC Biotechnology 2017 17:36

    Published on: 11 April 2017

  3. Research article

    Stable high-level expression of factor VIII in Chinese hamster ovary cells in improved elongation factor-1 alpha-based system

    Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which t...

    Nadezhda A. Orlova, Sergey V. Kovnir, Alexandre G. Gabibov and Ivan I. Vorobiev

    BMC Biotechnology 2017 17:33

    Published on: 24 March 2017

  4. Methodology article

    A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

    Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction s...

    Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, Jingao Dong and Yibin Lin

    BMC Biotechnology 2017 17:32

    Published on: 17 March 2017

  5. Research article

    Identification of residues important for the activity of aldehyde-deformylating oxygenase through investigation into the structure-activity relationship

    Aldehyde-deformylating oxygenase (ADO) is a key enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, cADO (cyanobacterial ADO) showed extreme low activity with the k ...

    Qing Wang, Luyao Bao, Chenjun Jia, Mei Li, Jian-Jun Li and Xuefeng Lu

    BMC Biotechnology 2017 17:31

    Published on: 16 March 2017

  6. Methodology article

    Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

    Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have...

    Jun-Zheng Wu, Qin Liu, Xiao-Shan Geng, Kai-Mian Li, Li-Juan Luo and Jin-Ping Liu

    BMC Biotechnology 2017 17:29

    Published on: 14 March 2017

  7. Research article

    The role of community engagement in the adoption of new agricultural biotechnologies by farmers: the case of the Africa harvest tissue-culture banana in Kenya

    The tissue culture banana (TCB) is a biotechnological agricultural innovation that has been adopted widely in commercial banana production. In 2003, Africa Harvest Biotech Foundation International (AH) initiat...

    Sunita V. S. Bandewar, Florence Wambugu, Emma Richardson and James V. Lavery

    BMC Biotechnology 2017 17:28

    Published on: 13 March 2017

  8. Research article

    An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites

    Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heat-labile...

    Nan Sun, Rongguang Zhang, Guangcai Duan, Xiaoyan Peng, Chen Wang, Qingtang Fan, Shuaiyin Chen and Yuanlin Xi

    BMC Biotechnology 2017 17:25

    Published on: 6 March 2017

  9. Research article

    Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization

    α-Amylase plays a pivotal role in a broad range of industrial processes. To meet increasing demands of biocatalytic tasks, considerable efforts have been made to isolate enzymes produced by extremophiles. Howe...

    Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, Siti Aisyah Alias, Bei Gao and Dongzhi Wei

    BMC Biotechnology 2017 17:22

    Published on: 28 February 2017

  10. Research article

    Constitutive expression of active microbial transglutaminase in Escherichia coli and comparative characterization to a known variant

    Microbial transglutaminase (mTG) is a robust enzyme catalyzing the formation of an isopeptide bond between glutamine and lysine residues. It has found use in food applications, pharmaceuticals, textiles, and b...

    Gabe Javitt, Zohar Ben-Barak-Zelas, Moran Jerabek-Willemsen and Ayelet Fishman

    BMC Biotechnology 2017 17:23

    Published on: 28 February 2017

  11. Research article

    Enhancing the thermostability of α-L-rhamnosidase from Aspergillus terreus and the enzymatic conversion of rutin to isoquercitrin by adding sorbitol

    Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method to improve the thermal stability...

    Lin Ge, Anna Chen, Jianjun Pei, Linguo Zhao, Xianying Fang, Gang Ding, Zhenzhong Wang, Wei Xiao and Feng Tang

    BMC Biotechnology 2017 17:21

    Published on: 27 February 2017

  12. Research article

    Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol

    Manganese peroxidase (MnP) of white rot basidiomycetes, an extracellular heme enzyme, is part of a peroxidase superfamily that is capable of degrading the different phenolic compounds. Ganoderma, a white rot basi...

    Hui Xu, Meng-Yuan Guo, Yan-Hua Gao, Xiao-Hui Bai and Xuan-Wei Zhou

    BMC Biotechnology 2017 17:19

    Published on: 23 February 2017

  13. Research article

    Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

    poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-sca...

    Jun Xia, Jiaxing Xu, Xiaoyan Liu, Jiming Xu, Xingfeng Wang and Xiangqian Li

    BMC Biotechnology 2017 17:20

    Published on: 23 February 2017

  14. Research article

    Evaluating the expression profile and stability of different UCOE containing vector combinations in mAb-producing CHO cells

    As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cel...

    Fatemeh Nematpour, Fereidoun Mahboudi, Behrouz Vaziri, Vahid Khalaj, Samira Ahmadi, Maryam Ahmadi, Saedeh Ebadat and Fatemeh Davami

    BMC Biotechnology 2017 17:18

    Published on: 22 February 2017

  15. Research article

    Retinoic acid promotes expression of germline-specific genes in chicken blastoderm cells by stimulating Smad1/5 phosphorylation in a feeder-free culture system

    Producing transgenic chickens with chicken blastodermal cells (cBCs) is inefficient due to the extremely low germline transmission capacity of cBCs. As chicken primordial germ cells (PGCs) have been reported a...

    Xiaochuan Tang, Shiyong Xu, Hongpeng Zhang, Qing Chen, Rongyang Li, Wangjun Wu, Minli Yu and Honglin Liu

    BMC Biotechnology 2017 17:17

    Published on: 20 February 2017

  16. Research article

    Penicillin production in industrial strain Penicillium chrysogenum P2niaD18 is not dependent on the copy number of biosynthesis genes

    Multi-copy gene integration into microbial genomes is a conventional tool for obtaining improved gene expression. For Penicillium chrysogenum, the fungal producer of the beta-lactam antibiotic penicillin, many pr...

    Sandra Ziemons, Katerina Koutsantas, Kordula Becker, Tim Dahlmann and Ulrich Kück

    BMC Biotechnology 2017 17:16

    Published on: 16 February 2017

  17. Research article

    Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

    Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-pol...

    Dede Abdulrachman, Paweena Thongkred, Kanokarn Kocharin, Monthon Nakpathom, Buppha Somboon, Nootsara Narumol, Verawat Champreda, Lily Eurwilaichitr, Antonius Suwanto, Thidarat Nimchua and Duriya Chantasingh

    BMC Biotechnology 2017 17:15

    Published on: 16 February 2017

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