One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester
© Bastings et al; licensee BioMed Central Ltd. 2008
Received: 20 February 2008
Accepted: 01 October 2008
Published: 01 October 2008
Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic groups or to immobilize proteins on surfaces and nanoparticles. Unfortunately, common refolding procedures for recombinant proteins that contain disulfide bonds do not preserve the thioester functionality and therefore novel refolding procedures need to be developed.
A novel redox buffer consisting of MESNA and diMESNA showed a refolding efficiency comparable to that of GSH/GSSG and prevented loss of the protein's thioester functionality. Moreover, introduction of the MESNA/diMESNA redox couple in the cleavage buffer allowed simultaneous on-column refolding of Ribonuclease A and intein-mediated cleavage to yield Ribonuclease A with a C-terminal MESNA-thioester. The C-terminal thioester was shown to be active in native chemical ligation.
An efficient method was developed for the production of disulfide bond containing proteins with C-terminal thioesters. Introduction of a MESNA/diMESNA redox couple resulted in simultaneous on-column refolding, purification and thioester generation of the model protein Ribonuclease A.
Most common methods for protein bioconjugation rely on the reaction of amines to activated esters or thiol groups to maleimides. The limited control over the specificity of these reactions results in heterogeneous protein conjugates and frequently gives rise to protein inactivation. A variety of bio-orthogonal ligation methods that were originally developed in peptide chemistry have recently been applied to allow for controlled protein conjugation. Native chemical ligation (NCL) is a chemoselective reaction of a C-terminal thioester with an N-terminal cysteine to yield a native peptide bond under aqueous conditions. This method was originally developed by Dawson et al. in 1994  to allow the chemical synthesis of proteins from smaller peptide fragments obtained by solid phase peptide synthesis [1–4]. The development of expression systems that use self-cleavable intein domains to generate recombinant proteins with a C-terminal thioester  resulted in a further extension of the possibilities for NCL. NCL has enabled the conjugation of recombinant proteins to spectroscopic labels, dendrimers, liposomes and micelles, as well as the site-specific immobilization of proteins to surfaces and the facile incorporation of unnatural amino acids [6–11]. Furthermore, posttranslational modifications that are inaccessible using standard E. coli expression systems can be introduced in recombinant proteins using NCL [12–14].
The C-terminal thioester that is required for NCL is easily obtained using the IMPACT™ system , which allows expression of the protein of interest as a fusion protein with a C-terminal intein domain followed by a chitin binding domain (CBD). Purification of the fusion protein using chitin affinity chromatography is typically followed by the addition of a small nucleophilic thiol, mercaptoethane sulphonic acid (MESNA). MESNA enables the cleavage of the target protein catalyzed by the intein domain, resulting in the production of a stable C-terminal thioester of the target protein. However, due to the reducing environment of the E. coli cytoplasm, disulfide bridges of recombinantly produced proteins are often not correctly formed. Refolding strategies for proteins with disulfide bonds typically use a glutathione redox buffer to assist in the refolding process [15–17]. We observed that incubation of proteins with a C-terminal MESNA – thioester with glutathione results in the formation of an unstable C-terminal glutathione-thioester, followed by hydrolysis to a carboxyl group.
Since the refolding procedure that is required to obtain disulfide containing proteins in their native state can lead to loss of the thioester functionality, NCL is typically performed directly on the immobilized fusion protein to yield a stable amide bond, followed by refolding in redox buffer systems [18, 19]. However, this method does not allow precise control of important ligation conditions such as reaction stoichiometry and it cannot be used to generate stable thioester proteins for subsequent conjugation to cysteine-functionalized surfaces. The alternative to perform protein refolding after ligation of the unfolded protein to a surface is less attractive, since most protein folding procedures yield substantial amount of misfolded proteins that are then also attached to the surface or nanoparticle.
Here we report the development of a new redox buffer based on MESNA and its oxidized disulfide 2,2-dithiobis(ethanesulfonate) (diMESNA) that shows a refolding efficiency that is comparable to that of the glutathione redox couple. Using Ribonuclease A as model protein we show that inclusion of diMESNA during intein-mediated cleavage results in simultaneous on column refolding and thioester formation, providing a novel method for the production of correctly folded, disulfide-bridge containing proteins with a C-terminal thioester suitable for native chemical ligation.
Results and discussion
Novel MESNA/diMESNA redox couple
Conjugation to Cys-PEG-DSPE
A novel procedure was developed that allows simultaneous purification, refolding and generation of a C-terminal thioester in recombinant proteins that contain disulfide bonds. The MESNA/diMESNA redox couple was shown to have a refolding efficiency that is comparable to that of the commonly used glutathione redox couple. The compatibility of the MESNA/diMESNA redox couple with the chitin column purification procedure for proteins with a C-terminal thioester was shown, resulting in on-column refolding simultaneously with cleavage of the protein from the intein domain and C-terminal thioester generation. This strategy should be generally applicable to generate disulfide-bond containing proteins with functional C-terminal thioesters for subsequent native chemical ligation.
Unless stated otherwise, all reagents and chemicals were obtained from commercial sources and used without further purification. Water was deionized prior to use. 6-FAM-dArUdAdA-6-TAMRA (Integrated DNA Technologies), 4-Mercaptophenylacetic-acid (MPAA) (Aldrich), Tris(2-carboxy-ethyl)phosphine hydrochloride (TCEP) (Sigma) were used as received. Ribonuclease A (grade XII-A, Sigma) was purified by reversed phase high pressure liquid chromatography (RP-HPLC) on a Varian Pro Star HPLC system coupled to a UV-Vis detector probing at 214 nm using a Vydac protein & peptide C18 column. A gradient of acetonitrile in water (both containing 0.1% trifluoroacetic acid (TFA)) was used to elute products. ESI-MS spectra were recorded on an Applied Biosystems Single Quadrupole Electrospray Ionization Mass Spectrometer API-150EX in positive or negative mode. Standard 1H NMR spectra were recorded at 25°C on a Varian Mercury 400 MHz spectrometer. Chemical shifts are given in ppm (δ) relative to tetramethylsilane (0 ppm). Fluorescence spectroscopy was performed on a Varian Cary Eclipse spectrometer at 10°C. All activity assays were performed in a 0.1 M Tris, 0.1 M NaCl, pH 8.0 buffer, with a substrate concentration of 400 nM.
GFP thioester exchange
GFP-MESNA  (10 μM) was incubated in either glutathione refolding buffer (0.1 M Na-PO4, pH 8 containing 3 mM reduced glutathione and 1 mM oxidized glutathione) or MESNA refolding buffer (0.1 M Na-PO4, pH 8, containing 3 mM MESNA and 1 mM diMESNA) After 10 h incubation at RT, samples were characterized using LC-MS.
RNase A (Sigma) was dissolved in assay buffer (0.1 M Tris, 0.1 M NaCl, pH 8.0) from a 40 μM stock. Upon addition of substrate (400 nM), fluorescence emission was monitored in time at 515 nm with excitation at 490 nm. The initial reaction rate v0 was calculated from a linear fit of the emission intensity in time over the first 30 seconds. A concentration range of RNase A was prepared and measured to obtain a calibration curve (1–10 nM; dilutions were freshly made before each measurement).
Synthesis of 2,2-dithiobis(ethanesulfonate) (diMESNA)
MESNA (3.28 g, 0.02 mol) was dissolved in water (100 mL) and added to a solution of 2,2 dithiobis(benzothiozole) (3.32 g, 0.01 mol) in chloroform (250 mL). The reaction mixture was stirred vigorously for 2 hours at room temperature. After separation of the organic layer, the water was evaporated and the remaining white solid was dissolved in methanol/water 8:2 (150 mL). Acetone was added till the cloud point, and the product precipitated as a white solid during 5 days at room temperature. After filtering, a mixture of diMESNA and MESNA was obtained as a white powder in 70% yield (2.30 g, 8.2 mmol). 1H-NMR showed the presence of 25% of MESNA in the product. Since MESNA was added in all experiments further purification was unnecessary.
1H-NMR (400 MHz, MeOD) diMESNA δ = 3.2-3.1 (m, 4H, SO3CH2CH2SSCH2CH2SO3), 3.1-3.0 (m, 4H SO3CH2CH2SSCH2CH2SO3), MESNA δ = 2.9-2.8 (m, 2H, SO3CH2CH2S), 3.1-3.0 (m, 2H, SO3CH2CH2S)
LC-MS: m/z [C4H10O6S4 - H]- Calcd. 280.94 Da; Obsd 281.0 Da; [2M - H]- Calcd 562.87 Da Obsd. 562.7 Da
Expression and purification of RNase A
The plasmid coding for RNase A-intein-CBD fusion protein, kindly provided by Prof. R. Raines (University of Wisconsin, Madison ), was transformed into chemically competent E. coli BL21(DE3) and plated on LB agar plates containing 100 mg L-1 ampicillin. Single colonies were used to inoculate 2 mL LB medium containing 100 mg L-1 ampicillin. Cultures were incubated overnight at 37°C and subsequently used to start 200 mL cultures containing 100 mg L-1 ampicillin. At OD600 = 0.5 the temperature was lowered to 15°C and 0.3 mM IPTG was added to induce expression of the target protein. After overnight expression at 15°C and 250 rpm cells were collected by centrifugation, resuspended in BugBuster lysis buffer (Novagen) and incubated for 20 minutes at 20°C. A clarified cell extract was obtained by centrifugation at 40,000 ×g for 45 min. The supernatant was loaded onto a 10 mL chitin column (New England Biolabs) that was equilibrated with 20 mM 3-(N-Morpholino)-propanesulfonic acid (MOPS) NaOH, 0.5 M NaCl, 0.1 mM EDTA, pH 6.8 (column buffer). The column was washed with 10 volumes of column buffer to remove non- and weak binding proteins. Subsequently, 3 volumes of cleavage buffer (50 mM MOPS NaOH, 0.5 M NaCl, 0.1 mM EDTA, 50 mM MESNA, pH 6.0) were flushed quickly through the column. After overnight incubation of the column at 20°C, the MESNA thioester of RNase A was eluted from the column using 1 volume of cleavage buffer. SDS-PAGE analysis of the eluted protein showed a single band at ~14 kDa.
ESI-MS: deconvoluted mass (oxidized) Calcd. 13995 Da, Obsd. 14000 Da.
Unfolding and refolding by dilution, glutathione vs MESNA redox couple
Unfolding: RNase A (0.465 mg, 33.9 nmol, SIGMA) was dissolved in 1 mL 4 M Gu·HCl, 100 mM TCEP. The mixture was incubated overnight at 20°C. Unfolding resulted in an increase of 8 Da from the 8 reduced cysteines.
ESI-MS: deconvoluted mass (reduced) Calcd. 13690 Da; Obsd. 13689 Da.
Refolding by dilution: Unfolded RNase A (500 μL) was added in 20 μL aliquots to 40 mL of refolding buffer (100 mM Tris·HCl, 100 mM NaCl; pH 8.0) containing either 3 mM reduced glutathione and 1 mM oxidized glutathione, or 3 mM MESNA and 1 mM diMESNA, and stirred overnight at room temperature. Refolding efficiencies were measured using the enzymatic activity assay at 5 nM initial RNase A concentration.
On-column refolding and protein purification
A 10 mL chitin column was equilibrated with 10 volumes (100 mL) of column buffer. The column was loaded with cell extract and washed with 10 volumes (100 mL) of column buffer. Subsequently, 2.5 volumes of refolding/cleavage buffer (50 mM MOPS NaOH, 0.5 M NaCl, 0.1 mM EDTA, 30 mM MESNA, 10 mM diMESNA, pH 6.0) were flushed quickly through the column. The column was incubated for three days at room temperature and the product was eluted from the column with 10 volumes of cleavage buffer (50 mM MOPS NaOH, 0.5 M NaCl, 0.1 mM EDTA, pH 6). Fractions were analyzed by SDS-PAGE and enzyme activity assay, giving an estimated yield of 0.6 mg L-1 active RNase A.
We thank Wencke Adriaens for performing the GFP-thioester exchange experiments. This work was supported by an NWO VIDI grant (700.56.428 to M.M) and the NRSC Catalysis
- Dawson PE, Muir TW, Clark-Lewis I, Kent SB: Synthesis of proteins by native chemical ligation. Science. 1994, 266: 776-779. 10.1126/science.7973629.View ArticleGoogle Scholar
- Hackeng TM, Rosing J, Spronk HMH, Vermeer C: Total chemical synthesis of human matrix Gla protein. Protein Sci. 2001, 10: 864-870. 10.1110/ps.44701.View ArticleGoogle Scholar
- Kent S: Total chemical synthesis of enzymes. J Pept Sci. 2003, 9: 574-593. 10.1002/psc.475.View ArticleGoogle Scholar
- Dawson PE, Kent SB: Synthesis of native proteins by chemical ligation. Annu Rev Biochem. 2000, 69: 923-960. 10.1146/annurev.biochem.69.1.923.View ArticleGoogle Scholar
- Muir TW, Sondhi D, Cole PA: Expressed protein ligation: A general method for protein engineering. Proc Natl Acad Sci USA. 1998, 95: 6705-6710. 10.1073/pnas.95.12.6705.View ArticleGoogle Scholar
- van Baal I, Malda H, Synowsky SA, van Dongen JLJ, Hackeng TM, Merkx M, Meijer EW: Multivalent peptide and protein dendrimers using native chemical ligation. Angew Chem, Int Ed. 2005, 44: 5052-5057. 10.1002/anie.200500635.View ArticleGoogle Scholar
- Reulen SWA, Brusselaars WWT, Langereis S, Mulder WJM, Breurken M, Merkx M: Protein-liposome conjugates using cysteine-lipids and Native Chemical Ligation. Bioconjugate Chem. 2007, 18: 590-596. 10.1021/bc0602782.View ArticleGoogle Scholar
- Lesaicherre ML, Lue RY, Chen GY, Zhu Q, Yao SQ: Intein-mediated biotinylation of proteins and its application in a protein microarray. J Am Chem Soc. 2002, 124: 8768-8769. 10.1021/ja0265963.View ArticleGoogle Scholar
- Gottlieb D, Grunwald C, Nowak C, Kuhlmann J, Waldmann H: Intein-mediated in vitro synthesis of lipidated Ras proteins. Chem Commun. 2006, 260-262. 10.1039/b511736d.Google Scholar
- Becker CF, Hunter CL, Seidel RP, Kent SB, Goody RS, Engelhard M: A sensitive fluorescence monitor for the detection of activated Ras: total chemical synthesis of site-specifically labeled Ras binding domain of c-Raf1 immobilized on a surface. Chem Biol. 2001, 8: 243-252. 10.1016/S1074-5521(01)00003-5.View ArticleGoogle Scholar
- Becker CF, Hunter CL, Seidel R, Kent SB, Goody RS, Engelhard M: Total chemical synthesis of a functional interacting protein pair: the protooncogene H-Ras and the Ras-binding domain of its effector c-Raf1. Proc Natl Acad Sci USA. 2003, 100: 5075-5080. 10.1073/pnas.0831227100.View ArticleGoogle Scholar
- Hackenberger CP, Friel CT, Radford SE, Imperiali B: Semisynthesis of a glycosylated Im7 analogue for protein folding studies. J Am Chem Soc. 2005, 127: 12882-12889. 10.1021/ja051855k.View ArticleGoogle Scholar
- Ball HL, King DS, Cohen FE, Prusiner SB, Baldwin MA: Engineering the prion protein using chemical synthesis. J Pept Res. 2001, 58: 357-374. 10.1034/j.1399-3011.2001.00943.x.View ArticleGoogle Scholar
- Macmillan D, Bertozzi CR: New directions in glycoprotein engineering. Tetrahedron. 2000, 56: 9515-9525. 10.1016/S0040-4020(00)00836-X.View ArticleGoogle Scholar
- Hevehan DL, De Bernardez Clark E: Oxidative renaturation of lysozyme at high concentrations. Biotechnol Bioeng. 1997, 54: 221-230. 10.1002/(SICI)1097-0290(19970505)54:3<221::AID-BIT3>3.0.CO;2-H.View ArticleGoogle Scholar
- Rudolph R, Lilie H: In vitro folding of inclusion body proteins. FASEB J. 1996, 10: 49-56.Google Scholar
- De Bernardez Clark E: Refolding of recombinant proteins. Curr Opin Biotechn. 1998, 9: 157-163. 10.1016/S0958-1669(98)80109-2.View ArticleGoogle Scholar
- Kalia J, Raines RT: Reactivity of intein thioesters: appending a functional group to a protein. Chem Bio Chem. 2006, 7: 1375-1383.View ArticleGoogle Scholar
- Sydor JR, Mariano M, Sideris S, Nock S: Establishment of Intein-Mediated Protein Ligation under Denaturing Conditions: C-Terminal Labeling of a Single-Chain Antibody for Biochip Screening. Bioconjugate Chem. 2002, 13: 707-712. 10.1021/bc025534z.View ArticleGoogle Scholar
- Brzezinska E: Disulfides. 1. Syntheses using 2,2'-dithiobis(benzothiazole). J Org Chem. 1994, 59: 8239-8244. 10.1021/jo00105a048.View ArticleGoogle Scholar
- Raines RT: Ribonuclease A. Chem Rev. 1998, 98: 1045-1065. 10.1021/cr960427h.View ArticleGoogle Scholar
- Kelemen BR, Klink TA, Behlke MA, Eubanks SR, Leland PA, Raines RT: Hypersensitive substrate for ribonucleases. Nucl Acid Res. 1999, 27: 3696-3701. 10.1093/nar/27.18.3696.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.