Figure 5

Optimization of cleavage and refolding conditions. Enzyme activity of cleaved RNase A-MESNA obtained after incubation for three days with different MESNA/diMESNA concentrations present in the cleavage buffer (50 mM MOPS, 500 mM NaCl, 0.1 mM EDTA, pH 6.0, at 20°C). Enzymatic activity measurements were performed in 0.1 M TRIS, 0.1 M NaCl, pH 8.0 buffer at 10°C with a substrate concentration of 400 nM.