Fig. 3From: Trigger factor assisted soluble expression of recombinant spike protein of porcine epidemic diarrhea virus in Escherichia coli Optimization of IPTG concentration (a), OD at induction (b) and harvest time (c) for soluble rGST-COE and rGST-S1D expression. The amount of soluble (S) and insoluble (I) fractions were compared relatively with each starting condition (IPTG = 0.1 mM, OD = 0.6, Time = 12 h), respectively, according to the band intensities of SDS-PAGE. The experiment was conducted twice independently, and the data from a representative experiment are shownBack to article page