Table 2 Biochemical characteristics of ChU-B mutant produced in P. pastoris and S. cerevisiae
From: Functional expression of a blood tolerant laccase in Pichia pastoris
Feature | ChU-B fromP. pastoris | ChU-B fromS. cerevisiae |
|---|---|---|
Expression levels (mg/L) | 43 | 8 |
MW1 | 53,939 | 53,939 |
MW2 | 62,541 | 60,310 |
Glycosylation (%) | 16 | 12 |
N-terminal end | ETEAEFSIGP | ETEAEFSIGP |
Optimum pH (with ABTS) | 4 | 4 |
Optimum pH (with DMP) | 5 | 5 |
T50 (°C) | 57.9 | 63.7 |