Figure 6

Location of the two mutations responsible for blood tolerance in the ChU-B mutant (B) compared with the corresponding residues in the parental type OB-1 (A). The F396I and F454E mutations are shown in yellow sticks and copper ions are depicted as blue spheres. The three residues in charge of the internal electron transfer from T1 Cu to T2/T3 cluster are displayed as magenta sticks. Residues involved in the first coordination sphere of the catalytic coppers and their interactions (green dashes) are also represented. The 3D-structure model is based on the crystal structure of the Trametes trogii laccase (97% identity, PDB: 2HRG) [33].