Figure 5From: High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coliHPLC analysis of purified rFGF21. The purity of rFGF21 eluted from a Ni-NTA sepharose column was further evaluated by HPLC analysis using a C18 column. As seen from the chromatogram, the y-axis indicates the absorbance, while the x-axis represents elution time (min). The main peak observed at 14.984 min corresponds to rFGF21.Back to article page