Fig. 2

The effect of temperature on AAV6.2FF infectivity in different buffer formulations. Aliquots of 1x10⁹ vg of AAV6.2FF-ffLuc formulated PBS or PBS supplemented with 0.001% PF-68 were exposed to a range of temperatures and durations. Following treatment, vector aliquots were applied to HEK293 cells and luciferase expression measured in relative luminescent units (RLU) 72 hours later. The temperatures tested were as follows: A -20℃, B 4℃, C 21℃, D 37℃, or E 55℃. A two-way ANOVA test was performed between the two buffer formulations in all exposure and treatment conditions. *P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001