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Fig. 2 | BMC Biotechnology

Fig. 2

From: Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves

Fig. 2

RYMVMg1ΔP1ΔCP/CterPSA RNA accumulation in Nicotiana benthamiana leaves. Semi-quantitative RT-PCR was performed on RNA extracted from N. benthamiana leaves infiltrated with either empty vector or RYMVMg1ΔP1ΔCP/CterPSA construct together with the cocktail of RNA silencing suppressors. Reverse transcriptions were performed using a mixture of oligodT and CterPSA specific primers. PCR amplification was performed using CterPSA-specific primers. NbEF1-α expression was used as internal control. Three independent experiments were performed. The results presented here are representative of the three experiments

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BMC Biotechnology

ISSN: 1472-6750

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