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Table 2 Plasmids used in this study

From: Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli

Plasmid

Assembly order

Plasmid

Assembly order

M-001

INPN-MerR

B-001

INPN-ChrB

M-002

FL-MerR

B-002

FL-ChrB

M-003

HL-MerR

B-003

HL-ChrB

M-004

RL-MerR

B-004

RL-ChrB

M-005

96 bp-MerR

B-005

96 bp-ChrB

M’-001

INPN-MerRopt

B’-001

INPN-ChrBopt

M’-002

FL-MerRopt

B’-002

FL-ChrBopt

M’-003

HL-MerRopt

B’-003

HL-ChrBopt

M’-004

RL- MerRopt

B’-004

RL- ChrBopt

M’-005

96 bp- MerRopt

B’-005

96 bp- ChrBopt

M-006

E-MerR-H

B-008

B-ChrB-E

M’-006

E -MerRopt-H

B’-008

B- ChrBopt-E

  1. E EcoRI, H HindIII, B BamHI